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An Agile Functional Analysis of Metagenomic Data Using SUPER-FOCUS.

Methods in molecular biology (Clifton, N.J.)

Silva GGZ, Lopes FAC, Edwards RA.
PMID: 28451970
Methods Mol Biol. 2017;1611:35-44. doi: 10.1007/978-1-4939-7015-5_4.

One of the main goals in metagenomics is to identify the functional profile of a microbial community from unannotated shotgun sequencing reads. Functional annotation is important in biological research because it enables researchers to identify the abundance of functional...

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Silva GGZ, Lopes FAC, Edwards RA. An Agile Functional Analysis of Metagenomic Data Using SUPER-FOCUS. Methods Mol Biol. 2017;1611:35-44doi: 10.1007/978-1-4939-7015-5_4.
Silva, G. G. Z., Lopes, F. A. C., & Edwards, R. A. (2017). An Agile Functional Analysis of Metagenomic Data Using SUPER-FOCUS. Methods in molecular biology (Clifton, N.J.), 161135-44. https://doi.org/10.1007/978-1-4939-7015-5_4
Silva, Genivaldo Gueiros Z, et al. "An Agile Functional Analysis of Metagenomic Data Using SUPER-FOCUS." Methods in molecular biology (Clifton, N.J.) vol. 1611 (2017): 35-44. doi: https://doi.org/10.1007/978-1-4939-7015-5_4
Silva GGZ, Lopes FAC, Edwards RA. An Agile Functional Analysis of Metagenomic Data Using SUPER-FOCUS. Methods Mol Biol. 2017;1611:35-44. doi: 10.1007/978-1-4939-7015-5_4. PMID: 28451970.
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Enzyme Annotation and Metabolic Reconstruction Using KEGG.

Methods in molecular biology (Clifton, N.J.)

Kanehisa M.
PMID: 28451977
Methods Mol Biol. 2017;1611:135-145. doi: 10.1007/978-1-4939-7015-5_11.

KEGG is an integrated database resource for linking sequences to biological functions from molecular to higher levels. Knowledge on molecular functions is stored in the KO (KEGG Orthology) database, while cellular- and organism-level functions are represented in the PATHWAY...

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Kanehisa M. Enzyme Annotation and Metabolic Reconstruction Using KEGG. Methods Mol Biol. 2017;1611:135-145doi: 10.1007/978-1-4939-7015-5_11.
Kanehisa, M. (2017). Enzyme Annotation and Metabolic Reconstruction Using KEGG. Methods in molecular biology (Clifton, N.J.), 1611135-145. https://doi.org/10.1007/978-1-4939-7015-5_11
Kanehisa, Minoru. "Enzyme Annotation and Metabolic Reconstruction Using KEGG." Methods in molecular biology (Clifton, N.J.) vol. 1611 (2017): 135-145. doi: https://doi.org/10.1007/978-1-4939-7015-5_11
Kanehisa M. Enzyme Annotation and Metabolic Reconstruction Using KEGG. Methods Mol Biol. 2017;1611:135-145. doi: 10.1007/978-1-4939-7015-5_11. PMID: 28451977.
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Rat Hepatocyte Cultures : Conventional Monolayer and Cocultures With Rat Liver Epithelial Cells.

Methods in molecular biology (Clifton, N.J.)

Henkens T, Vanhaecke T, Papeleu P, Elaut G, Vinken M, Snykers S, Rogiers V.
PMID: 27699670
Methods Mol Biol. 2006;320:239-246. doi: 10.1385/1-59259-998-2:239.

Primary cultures of hepatocytes are useful tools for both short- and long-term pharmacotoxicological research. Under conventional conditions, isolated hepatocytes form a monolayer and survive for about 1 wk but lose some liver-specific functions, including xenobiotic biotransformation. In comparison with...

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Henkens T, Vanhaecke T, Papeleu P, et al. Rat Hepatocyte Cultures : Conventional Monolayer and Cocultures With Rat Liver Epithelial Cells. Methods Mol Biol. 2006;320:239-246doi: 10.1385/1-59259-998-2:239.
Henkens, T., Vanhaecke, T., Papeleu, P., Elaut, G., Vinken, M., Snykers, S., & Rogiers, V. (2006). Rat Hepatocyte Cultures : Conventional Monolayer and Cocultures With Rat Liver Epithelial Cells. Methods in molecular biology (Clifton, N.J.), 320239-246. https://doi.org/10.1385/1-59259-998-2:239
Henkens, Tom, et al. "Rat Hepatocyte Cultures : Conventional Monolayer and Cocultures With Rat Liver Epithelial Cells." Methods in molecular biology (Clifton, N.J.) vol. 320 (2006): 239-246. doi: https://doi.org/10.1385/1-59259-998-2:239
Henkens T, Vanhaecke T, Papeleu P, Elaut G, Vinken M, Snykers S, Rogiers V. Rat Hepatocyte Cultures : Conventional Monolayer and Cocultures With Rat Liver Epithelial Cells. Methods Mol Biol. 2006;320:239-246. doi: 10.1385/1-59259-998-2:239. PMID: 27699670.
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Deletion of Genes From the Mouse Genome Using Cre/loxP Technology.

Methods in molecular biology (Clifton, N.J.)

Hernandez D, Chandan P, Janmohamed A, Phillips IR, Shephard EA.
PMID: 27699674
Methods Mol Biol. 2006;320:307-319. doi: 10.1385/1-59259-998-2:307.

The steps required to delete genes from the mouse genome are illustrated by showing how a cluster of three flavin-containing monooxygenase (Fmo) genes (Fmo1, Fmo2, and Fmo4) were deleted from mouse chromosome 1. Such large deletions are accomplished using...

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Hernandez D, Chandan P, Janmohamed A, et al. Deletion of Genes From the Mouse Genome Using Cre/loxP Technology. Methods Mol Biol. 2006;320:307-319doi: 10.1385/1-59259-998-2:307.
Hernandez, D., Chandan, P., Janmohamed, A., Phillips, I. R., & Shephard, E. A. (2006). Deletion of Genes From the Mouse Genome Using Cre/loxP Technology. Methods in molecular biology (Clifton, N.J.), 320307-319. https://doi.org/10.1385/1-59259-998-2:307
Hernandez, Diana, et al. "Deletion of Genes From the Mouse Genome Using Cre/loxP Technology." Methods in molecular biology (Clifton, N.J.) vol. 320 (2006): 307-319. doi: https://doi.org/10.1385/1-59259-998-2:307
Hernandez D, Chandan P, Janmohamed A, Phillips IR, Shephard EA. Deletion of Genes From the Mouse Genome Using Cre/loxP Technology. Methods Mol Biol. 2006;320:307-319. doi: 10.1385/1-59259-998-2:307. PMID: 27699674.
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Expression of Recombinant Flavin-Containing Monooxygenases in a Baculovirus/Insect Cell System.

Methods in molecular biology (Clifton, N.J.)

Janmohamed A, Thaunsukon P, Shephard EA, Phillips IR.
PMID: 27699678
Methods Mol Biol. 2006;320:39-59. doi: 10.1385/1-59259-998-2:39.

The baculovirus/insect cell heterologous expression system provides an important tool for investigating the catalytic activity of individual drug-metabolizing enzymes toward a particular substrate. In this chapter we describe a baculovirus/insect cell system that we have used for the expression...

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Janmohamed A, Thaunsukon P, Shephard EA, et al. Expression of Recombinant Flavin-Containing Monooxygenases in a Baculovirus/Insect Cell System. Methods Mol Biol. 2006;320:39-59doi: 10.1385/1-59259-998-2:39.
Janmohamed, A., Thaunsukon, P., Shephard, E. A., & Phillips, I. R. (2006). Expression of Recombinant Flavin-Containing Monooxygenases in a Baculovirus/Insect Cell System. Methods in molecular biology (Clifton, N.J.), 32039-59. https://doi.org/10.1385/1-59259-998-2:39
Janmohamed, Azara, et al. "Expression of Recombinant Flavin-Containing Monooxygenases in a Baculovirus/Insect Cell System." Methods in molecular biology (Clifton, N.J.) vol. 320 (2006): 39-59. doi: https://doi.org/10.1385/1-59259-998-2:39
Janmohamed A, Thaunsukon P, Shephard EA, Phillips IR. Expression of Recombinant Flavin-Containing Monooxygenases in a Baculovirus/Insect Cell System. Methods Mol Biol. 2006;320:39-59. doi: 10.1385/1-59259-998-2:39. PMID: 27699678.
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High-Performance Liquid Chromatography Analysis of CYP2C8-Catalyzed Paclitaxel 6α-Hydroxylation.

Methods in molecular biology (Clifton, N.J.)

Crespi CL, Chang TK, Waxman DJ.
PMID: 27699666
Methods Mol Biol. 2006;320:103-107. doi: 10.1385/1-59259-998-2:103.

Paclitaxel (Taxol) is a naturally occurring member of the taxane family of antitumor drugs, which act by stabilizing microtubules. Paclitaxel is inactivated in human liver by a cytochrome P450 (P450)-catalyzed 6α-hydroxylation reaction. A reverse-phase, high-performance liquid chromatographic assay is...

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Crespi CL, Chang TK, Waxman DJ. High-Performance Liquid Chromatography Analysis of CYP2C8-Catalyzed Paclitaxel 6α-Hydroxylation. Methods Mol Biol. 2006;320:103-107doi: 10.1385/1-59259-998-2:103.
Crespi, C. L., Chang, T. K., & Waxman, D. J. (2006). High-Performance Liquid Chromatography Analysis of CYP2C8-Catalyzed Paclitaxel 6α-Hydroxylation. Methods in molecular biology (Clifton, N.J.), 320103-107. https://doi.org/10.1385/1-59259-998-2:103
Crespi, Charles L, et al. "High-Performance Liquid Chromatography Analysis of CYP2C8-Catalyzed Paclitaxel 6α-Hydroxylation." Methods in molecular biology (Clifton, N.J.) vol. 320 (2006): 103-107. doi: https://doi.org/10.1385/1-59259-998-2:103
Crespi CL, Chang TK, Waxman DJ. High-Performance Liquid Chromatography Analysis of CYP2C8-Catalyzed Paclitaxel 6α-Hydroxylation. Methods Mol Biol. 2006;320:103-107. doi: 10.1385/1-59259-998-2:103. PMID: 27699666.
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Erratum to: Colorimetric Detection of Acetyl Xylan Esterase Activities.

Methods in molecular biology (Clifton, N.J.)

Mai-Gisondi G, Master ER.
PMID: 28597289
Methods Mol Biol. 2017;1588:E1-E3. doi: 10.1007/978-1-4939-6899-2_23.

No abstract available.

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Mai-Gisondi G, Master ER. Erratum to: Colorimetric Detection of Acetyl Xylan Esterase Activities. Methods Mol Biol. 2017;1588:E1-E3doi: 10.1007/978-1-4939-6899-2_23.
Mai-Gisondi, G., & Master, E. R. (2017). Erratum to: Colorimetric Detection of Acetyl Xylan Esterase Activities. Methods in molecular biology (Clifton, N.J.), 1588E1-E3. https://doi.org/10.1007/978-1-4939-6899-2_23
Mai-Gisondi, Galina, and Master, Emma R. "Erratum to: Colorimetric Detection of Acetyl Xylan Esterase Activities." Methods in molecular biology (Clifton, N.J.) vol. 1588 (2017): E1-E3. doi: https://doi.org/10.1007/978-1-4939-6899-2_23
Mai-Gisondi G, Master ER. Erratum to: Colorimetric Detection of Acetyl Xylan Esterase Activities. Methods Mol Biol. 2017;1588:E1-E3. doi: 10.1007/978-1-4939-6899-2_23. PMID: 28597289.
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Erratum to: In Vivo Model of Small Intestine.

Methods in molecular biology (Clifton, N.J.)

Mahe MM, Brown NE, Poling HM, Helmrath MA.
PMID: 28667364
Methods Mol Biol. 2017;1597:E1. doi: 10.1007/978-1-4939-6949-4_18.

No abstract available.

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Mahe MM, Brown NE, Poling HM, et al. Erratum to: In Vivo Model of Small Intestine. Methods Mol Biol. 2017;1597:E1doi: 10.1007/978-1-4939-6949-4_18.
Mahe, M. M., Brown, N. E., Poling, H. M., & Helmrath, M. A. (2017). Erratum to: In Vivo Model of Small Intestine. Methods in molecular biology (Clifton, N.J.), 1597E1. https://doi.org/10.1007/978-1-4939-6949-4_18
Mahe, Maxime M, et al. "Erratum to: In Vivo Model of Small Intestine." Methods in molecular biology (Clifton, N.J.) vol. 1597 (2017): E1. doi: https://doi.org/10.1007/978-1-4939-6949-4_18
Mahe MM, Brown NE, Poling HM, Helmrath MA. Erratum to: In Vivo Model of Small Intestine. Methods Mol Biol. 2017;1597:E1. doi: 10.1007/978-1-4939-6949-4_18. PMID: 28667364.
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Production and analytic bioinformatics for next-generation DNA sequencing.

Methods in molecular biology (Clifton, N.J.)

Allcock RJ.
PMID: 24870128
Methods Mol Biol. 2014;1168:17-29. doi: 10.1007/978-1-4939-0847-9_2.

The bioinformatics requirements within the clinical environment are very specific, and analytic techniques need to be fit for purpose, robust, and predictable. At the same time, the bewildering amount of information produced during these analyses needs to be carefully...

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Allcock RJ. Production and analytic bioinformatics for next-generation DNA sequencing. Methods Mol Biol. 2014;1168:17-29doi: 10.1007/978-1-4939-0847-9_2.
Allcock, R. J. (2014). Production and analytic bioinformatics for next-generation DNA sequencing. Methods in molecular biology (Clifton, N.J.), 116817-29. https://doi.org/10.1007/978-1-4939-0847-9_2
Allcock, Richard James Nigel. "Production and analytic bioinformatics for next-generation DNA sequencing." Methods in molecular biology (Clifton, N.J.) vol. 1168 (2014): 17-29. doi: https://doi.org/10.1007/978-1-4939-0847-9_2
Allcock RJ. Production and analytic bioinformatics for next-generation DNA sequencing. Methods Mol Biol. 2014;1168:17-29. doi: 10.1007/978-1-4939-0847-9_2. PMID: 24870128.
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Erratum to: Methods for analysis of AP-3/Rabin4' in regulation of lysosome distribution.

Methods in molecular biology (Clifton, N.J.)

Ivan V, van der Sluijs P.
PMID: 25896102
Methods Mol Biol. 2015;1298:E1. doi: 10.1007/978-1-4939-2569-8_29.

No abstract available.

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Ivan V, van der Sluijs P. Erratum to: Methods for analysis of AP-3/Rabin4' in regulation of lysosome distribution. Methods Mol Biol. 2015;1298:E1doi: 10.1007/978-1-4939-2569-8_29.
Ivan, V., & van der Sluijs, P. (2015). Erratum to: Methods for analysis of AP-3/Rabin4' in regulation of lysosome distribution. Methods in molecular biology (Clifton, N.J.), 1298E1. https://doi.org/10.1007/978-1-4939-2569-8_29
Ivan, Viorica, and van der Sluijs, Peter. "Erratum to: Methods for analysis of AP-3/Rabin4' in regulation of lysosome distribution." Methods in molecular biology (Clifton, N.J.) vol. 1298 (2015): E1. doi: https://doi.org/10.1007/978-1-4939-2569-8_29
Ivan V, van der Sluijs P. Erratum to: Methods for analysis of AP-3/Rabin4' in regulation of lysosome distribution. Methods Mol Biol. 2015;1298:E1. doi: 10.1007/978-1-4939-2569-8_29. PMID: 25896102.
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Computationally guided design of robust gene circuits.

Methods in molecular biology (Clifton, N.J.)

Shah NA, Sarkar CA.
PMID: 25487097
Methods Mol Biol. 2015;1244:167-78. doi: 10.1007/978-1-4939-1878-2_8.

The inability to rationally design and construct circuits that robustly enable complex behaviors is perhaps the most fundamental challenge in synthetic biology. While systems modeling can aid this process and help reduce the space of design strategies, the unavailability...

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Shah NA, Sarkar CA. Computationally guided design of robust gene circuits. Methods Mol Biol. 2015;1244:167-78doi: 10.1007/978-1-4939-1878-2_8.
Shah, N. A., & Sarkar, C. A. (2015). Computationally guided design of robust gene circuits. Methods in molecular biology (Clifton, N.J.), 1244167-78. https://doi.org/10.1007/978-1-4939-1878-2_8
Shah, Najaf A, and Sarkar, Casim A. "Computationally guided design of robust gene circuits." Methods in molecular biology (Clifton, N.J.) vol. 1244 (2015): 167-78. doi: https://doi.org/10.1007/978-1-4939-1878-2_8
Shah NA, Sarkar CA. Computationally guided design of robust gene circuits. Methods Mol Biol. 2015;1244:167-78. doi: 10.1007/978-1-4939-1878-2_8. PMID: 25487097.
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Erratum to: Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression.

Methods in molecular biology (Clifton, N.J.)

Ochoa-Fernandez R, Samodelov SL, Brandl SM, Wehinger E, Müller K, Weber W, Zurbriggen MD.
PMID: 27314667
Methods Mol Biol. 2016;1408:E1. doi: 10.1007/978-1-4939-3512-3_28.

No abstract available.

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Ochoa-Fernandez R, Samodelov SL, Brandl SM, et al. Erratum to: Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression. Methods Mol Biol. 2016;1408:E1doi: 10.1007/978-1-4939-3512-3_28.
Ochoa-Fernandez, R., Samodelov, S. L., Brandl, S. M., Wehinger, E., Müller, K., Weber, W., & Zurbriggen, M. D. (2016). Erratum to: Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression. Methods in molecular biology (Clifton, N.J.), 1408E1. https://doi.org/10.1007/978-1-4939-3512-3_28
Ochoa-Fernandez, Rocio, et al. "Erratum to: Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression." Methods in molecular biology (Clifton, N.J.) vol. 1408 (2016): E1. doi: https://doi.org/10.1007/978-1-4939-3512-3_28
Ochoa-Fernandez R, Samodelov SL, Brandl SM, Wehinger E, Müller K, Weber W, Zurbriggen MD. Erratum to: Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression. Methods Mol Biol. 2016;1408:E1. doi: 10.1007/978-1-4939-3512-3_28. PMID: 27314667.
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Showing 1 to 12 of 3397 entries